1I7A

EVH1 DOMAIN FROM MURINE HOMER 2B/VESL 2

1I7A の概要

• エントリーDOI: 10.2210/pdb1i7a/pdb
• 関連するPDBエントリー: 1ddv 1ddw
• 分子名称: HOMER 2B, PHE-ALA-PHE, CITRATE ANION, ... (5 entities in total)
• 機能のキーワード: evh1 domain, homer, vesl, x-ray crystal structure, brain, signaling protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Cytoplasm : Q9QWW1
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 51561.98

構造登録者

Barzik, M.,Carl, U.D.,Schubert, W.-D.,Wehland, J.,Heinz, D.W. (登録日: 2001-03-08, 公開日: 2001-08-22, 最終更新日: 2023-08-09)

主引用文献

Barzik, M.,Carl, U.D.,Schubert, W.D.,Frank, R.,Wehland, J.,Heinz, D.W.
The N-terminal domain of Homer/Vesl is a new class II EVH1 domain.
J.Mol.Biol., 309:155-169, 2001

PubMed Abstract: Cellular activities controlled by signal transduction processes such as cell motility and cell growth depend on the tightly regulated assembly of multiprotein complexes. Adapter proteins that specifically interact with their target proteins are key components required for the formation of these assemblies. Ena/VASP-homology 1 (EVH1) domains are small constituents of large modular proteins involved in microfilament assembly that specifically recognize proline-rich regions. EVH1 domain-containing proteins are present in neuronal cells, like the Homer/Vesl protein family that is involved in memory-generating processes. Here, we describe the crystal structure of the murine EVH1 domain of Vesl 2 at 2.2 A resolution. The small globular protein consists of a seven-stranded antiparallel beta-barrel with a C-terminal alpha-helix packing alongside the barrel. A shallow groove running parallel with beta-strand VI forms an extended peptide-binding site. Using peptide library screenings, we present data that demonstrate the high affinity of the Vesl 2 EVH1 domain towards peptide sequences containing a proline-rich core sequence (PPSPF) that requires additional charged amino acid residues on either side for specific binding. Our functional data, substantiated by structural data, demonstrate that the ligand-binding of the Vesl EVH1 domain differs from the interaction characteristics of the previously examined EVH1 domains of the Evl/Mena proteins. Analogous to the Src homology 3 (SH3) domains that bind their cognate ligands in two distinct directions, we therefore propose the existence of two distinct classes of EVH1 domains.

PubMed: 11491285
DOI: 10.1006/jmbi.2001.4640

実験手法

X-RAY DIFFRACTION (2.24 Å)