1I5S

CRYSTAL STRUCTURE OF THE KIF1A MOTOR DOMAIN COMPLEXED WITH MG-ADP

1I5S の概要

• エントリーDOI: 10.2210/pdb1i5s/pdb
• 分子名称: KINESIN-LIKE PROTEIN KIF1A, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: kinesin catalytic core, motor domain, transport protein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm, cytoskeleton (Probable): P33173
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41691.83

構造登録者

Kikkawa, M.,Sablin, E.P.,Okada, Y.,Yajima, H.,Fletterick, R.J.,Hirokawa, N. (登録日: 2001-02-28, 公開日: 2001-05-30, 最終更新日: 2024-04-03)

主引用文献

Kikkawa, M.,Sablin, E.P.,Okada, Y.,Yajima, H.,Fletterick, R.J.,Hirokawa, N.
Switch-based mechanism of kinesin motors
Nature, 411:439-445, 2001

PubMed Abstract: Kinesin motors are specialized enzymes that use hydrolysis of ATP to generate force and movement along their cellular tracks, the microtubules. Although numerous biochemical and biophysical studies have accumulated much data that link microtubule-assisted ATP hydrolysis to kinesin motion, the structural view of kinesin movement remains unclear. This study of the monomeric kinesin motor KIF1A combines X-ray crystallography and cryo-electron microscopy, and allows analysis of force-generating conformational changes at atomic resolution. The motor is revealed in its two functionally critical states-complexed with ADP and with a non-hydrolysable analogue of ATP. The conformational change observed between the ADP-bound and the ATP-like structures of the KIF1A catalytic core is modular, extends to all kinesins and is similar to the conformational change used by myosin motors and G proteins. Docking of the ADP-bound and ATP-like crystallographic models of KIF1A into the corresponding cryo-electron microscopy maps suggests a rationale for the plus-end directional bias associated with the kinesin catalytic core.

PubMed: 11373668
DOI: 10.1038/35078000

実験手法

X-RAY DIFFRACTION (2.2 Å)