1I50

RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION

1I50 の概要

• エントリーDOI: 10.2210/pdb1i50/pdb
• 関連するPDBエントリー: 1EN0 1I3Q
• 分子名称: DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT, DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE, ZINC ION, ... (13 entities in total)
• 機能のキーワード: transcription, mrna, multiprotein complex, molecular machine, zinc motifs
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P08518 P16370 P20434 P20436 P38902; Nucleus, nucleolus : P40422 P27999 P22139; Cytoplasm : P20435
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 470204.19

構造登録者

Cramer, P.,Bushnell, D.A.,Kornberg, R.D. (登録日: 2001-02-23, 公開日: 2001-04-23, 最終更新日: 2024-02-07)

主引用文献

Cramer, P.,Bushnell, D.A.,Kornberg, R.D.
Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution.
Science, 292:1863-1876, 2001

PubMed Abstract: Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.

PubMed: 11313498
DOI: 10.1126/science.1059493

実験手法

X-RAY DIFFRACTION (2.8 Å)