1I4W

THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION

1I4W の概要

• エントリーDOI: 10.2210/pdb1i4w/pdb
• 分子名称: MITOCHONDRIAL REPLICATION PROTEIN MTF1, XENON (3 entities in total)
• 機能のキーワード: mitochondrial transcription factor, transcription initiation, transcription
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41488.03

構造登録者

Schubot, F.D.,Chen, C.-J.,Rose, J.P.,Dailey, T.A.,Dailey, H.A.,Wang, B.-C. (登録日: 2001-02-23, 公開日: 2001-10-03, 最終更新日: 2024-02-07)

主引用文献

Schubot, F.D.,Chen, C.J.,Rose, J.P.,Dailey, T.A.,Dailey, H.A.,Wang, B.C.
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription.
Protein Sci., 10:1980-1988, 2001

PubMed Abstract: Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site.

PubMed: 11567089
DOI: 10.1110/ps.11201

実験手法

X-RAY DIFFRACTION (2.6 Å)