1I4S

CRYSTAL STRUCTURE OF RNASE III ENDONUCLEASE DOMAIN FROM AQUIFEX AEOLICUS AT 2.15 ANGSTROM RESOLUTION

1I4S の概要

• エントリーDOI: 10.2210/pdb1i4s/pdb
• 関連するPDBエントリー: 1DI2 1STU
• 分子名称: RIBONUCLEASE III (2 entities in total)
• 機能のキーワード: ribonuclease, rnase iii, double-stranded rna, catalytic domain, endonuclease domain, endonucleolytic cleavage, hydrolase
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm : O67082
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34554.16

構造登録者

Blaszczyk, J.,Ji, X. (登録日: 2001-02-22, 公開日: 2001-12-19, 最終更新日: 2023-08-30)

主引用文献

Blaszczyk, J.,Tropea, J.E.,Bubunenko, M.,Routzahn, K.M.,Waugh, D.S.,Court, D.L.,Ji, X.
Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.
Structure, 9:1225-1236, 2001

PubMed Abstract: Aquifex aeolicus Ribonuclease III (Aa-RNase III) belongs to the family of Mg(2+)-dependent endonucleases that show specificity for double-stranded RNA (dsRNA). RNase III is conserved in all known bacteria and eukaryotes and has 1-2 copies of a 9-residue consensus sequence, known as the RNase III signature motif. The bacterial RNase III proteins are the simplest, consisting of two domains: an N-terminal endonuclease domain, followed by a double-stranded RNA binding domain (dsRBD). The three-dimensional structure of the dsRBD in Escherichia coli RNase III has been elucidated; no structural information is available for the endonuclease domain of any RNase III.

PubMed: 11738048
DOI: 10.1016/S0969-2126(01)00685-2

実験手法

X-RAY DIFFRACTION (2.15 Å)