1I3T

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA

1I3T の概要

• エントリーDOI: 10.2210/pdb1i3t/pdb
• 分子名称: 5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*(C45)P*GP*CP*GP)-3', MAGNESIUM ION (3 entities in total)
• 機能のキーワード: n4-methoxycytosine, damaged dna, b-dna, double helix, deoxyribonucleic acid, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7411.14

構造登録者

Hossain, M.T.,Hikima, T.,Chatake, T.,Masaru, T.,Sunami, T.,Ueno, Y.,Matsuda, A.,Takenaka, A. (登録日: 2001-02-16, 公開日: 2001-09-14, 最終更新日: 2023-08-09)

主引用文献

Hossain, M.T.,Chatake, T.,Hikima, T.,Tsunoda, M.,Sunami, T.,Ueno, Y.,Matsuda, A.,Takenaka, A.
Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex.
J.Biochem.(Tokyo), 130:9-12, 2001

PubMed Abstract: To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.

PubMed: 11432773

実験手法

X-RAY DIFFRACTION (1.6 Å)