1I2M

RAN-RCC1-SO4 COMPLEX

1I2M の概要

• エントリーDOI: 10.2210/pdb1i2m/pdb
• 関連するPDBエントリー: 1a12 1BYU 1RRP
• 分子名称: GTP-BINDING NUCLEAR PROTEIN RAN, REGULATOR OF CHROMOSOME CONDENSATION 1, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: beta-propeller, g fold or gtpase fold, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P62826 P18754
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 134939.14

構造登録者

Renault, L.,Kuhlmann, J.,Henkel, A.,Wittinghofer, A. (登録日: 2001-02-11, 公開日: 2001-05-02, 最終更新日: 2023-08-09)

主引用文献

Renault, L.,Kuhlmann, J.,Henkel, A.,Wittinghofer, A.
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).
Cell(Cambridge,Mass.), 105:245-255, 2001

PubMed Abstract: RCC1 (regulator of chromosome condensation), a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, an intermediate in the multistep GEF reaction. In contrast to previous structures, the phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop. Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of the P loop lysine with an acidic residue is a crucial element for the overall reaction.

PubMed: 11336674
DOI: 10.1016/S0092-8674(01)00315-4

実験手法

X-RAY DIFFRACTION (1.76 Å)