1I27

CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)

1I27 の概要

• エントリーDOI: 10.2210/pdb1i27/pdb
• 分子名称: TRANSCRIPTION FACTOR IIF, ZINC ION (3 entities in total)
• 機能のキーワード: general transcription factor, rap74, rap30, tfiif, rna polymerase ii, winged-helix domain, transcription
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8440.21

構造登録者

Kamada, K.,De Angelis, J.,Roeder, R.G.,Burley, S.K. (登録日: 2001-02-07, 公開日: 2001-03-07, 最終更新日: 2024-02-07)

主引用文献

Kamada, K.,De Angelis, J.,Roeder, R.G.,Burley, S.K.
Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF.
Proc.Natl.Acad.Sci.USA, 98:3115-3120, 2001

PubMed Abstract: The x-ray structure of a C-terminal fragment of the RAP74 subunit of human transcription factor (TF) IIF has been determined at 1.02-A resolution. The alpha/beta structure is strikingly similar to the globular domain of linker histone H5 and the DNA-binding domain of hepatocyte nuclear factor 3gamma (HNF-3gamma), making it a winged-helix protein. The surface electrostatic properties of this compact domain differ significantly from those of bona fide winged-helix transcription factors (HNF-3gamma and RFX1) and from the winged-helix domains found within the RAP30 subunit of TFIIF and the beta subunit of TFIIE. RAP74 has been shown to interact with the TFIIF-associated C-terminal domain phosphatase FCP1, and a putative phosphatase binding site has been identified within the RAP74 winged-helix domain.

PubMed: 11248041
DOI: 10.1073/pnas.051631098

実験手法

X-RAY DIFFRACTION (1.02 Å)