1HWQ

SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP

1HWQ の概要

• エントリーDOI: 10.2210/pdb1hwq/pdb
• NMR情報: BMRB: 5007
• 分子名称: VS RIBOZYME SUBSTRATE RNA (1 entity in total)
• 機能のキーワード: vs ribozyme, stem-loop, tandem ga, protonated adenine, a+c base pair, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9705.83

構造登録者

Flinders, J.C.,Dieckmann, T. (登録日: 2001-01-09, 公開日: 2001-05-16, 最終更新日: 2024-05-22)

主引用文献

Flinders, J.,Dieckmann, T.
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA.
J.Mol.Biol., 308:665-679, 2001

PubMed Abstract: The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.

PubMed: 11350168
DOI: 10.1006/jmbi.2001.4627

実験手法

SOLUTION NMR