1HV2

SOLUTION STRUCTURE OF YEAST ELONGIN C IN COMPLEX WITH A VON HIPPEL-LINDAU PEPTIDE

1HV2 の概要

• エントリーDOI: 10.2210/pdb1hv2/pdb
• 分子名称: ELONGIN C, VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR (2 entities in total)
• 機能のキーワード: protein-peptide complex, signaling protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13114.95

構造登録者

Botuyan, M.V.,Mer, G.,Yi, G.-S.,Koth, C.M.,Case, D.A.,Edwards, A.M.,Chazin, W.J.,Arrowsmith, C.H. (登録日: 2001-01-05, 公開日: 2001-09-06, 最終更新日: 2024-05-22)

主引用文献

Botuyan, M.V.,Mer, G.,Yi, G.S.,Koth, C.M.,Case, D.A.,Edwards, A.M.,Chazin, W.J.,Arrowsmith, C.H.
Solution structure and dynamics of yeast elongin C in complex with a von Hippel-Lindau peptide.
J.Mol.Biol., 312:177-186, 2001

PubMed Abstract: Elongin is a transcription elongation factor that stimulates the rate of elongation by suppressing transient pausing by RNA polymerase II at many sites along the DNA. It is heterotrimeric in mammals, consisting of elongins A, B and C subunits, and bears overall similarity to a class of E3 ubiquitin ligases known as SCF (Skp1-Cdc53 (cullin)-F-box) complexes. A subcomplex of elongins B and C is a target for negative regulation by the von Hippel-Lindau (VHL) tumor-suppressor protein. Elongin C from Saccharomyces cerevisiae, Elc1, exhibits high sequence similarity to mammalian elongin C. Using NMR spectroscopy we have determined the three-dimensional structure of Elc1 in complex with a human VHL peptide, VHL(157-171), representing the major Elc1 binding site. The bound VHL peptide is entirely helical. Elc1 utilizes two C-terminal helices and an intervening loop to form a binding groove that fits VHL(157-171). Chemical shift perturbation and dynamics analyses reveal that a global conformational change accompanies Elc1/VHL(157-171) complex formation. Moreover, the disappearance of conformational exchange phenomena on the microsecond to millisecond time scale within Elc1 upon VHL peptide binding suggests a role for slow internal motions in ligand recognition.

PubMed: 11545595
DOI: 10.1006/jmbi.2001.4938

実験手法

SOLUTION NMR