1HU3

MIDDLE DOMAIN OF HUMAN EIF4GII

1HU3 の概要

• エントリーDOI: 10.2210/pdb1hu3/pdb
• 分子名称: EIF4GII (2 entities in total)
• 機能のキーワード: eif4g, heat repeat, translation
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30826.41

構造登録者

Marcotrigiano, J.,Lomakin, I.,Pestova, T.V.,Hellen, C.U.T.,Sonenberg, N.,Burley, S.K. (登録日: 2001-01-03, 公開日: 2001-02-21, 最終更新日: 2024-10-30)

主引用文献

Marcotrigiano, J.,Lomakin, I.B.,Sonenberg, N.,Pestova, T.V.,Hellen, C.U.,Burley, S.K.
A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery.
Mol.Cell, 7:193-203, 2001

PubMed Abstract: The X-ray structure of the phylogenetically conserved middle portion of human eukaryotic initiation factor (eIF) 4GII has been determined at 2.4 A resolution, revealing a crescent-shaped domain consisting of ten alpha helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation.

PubMed: 11172724
DOI: 10.1016/S1097-2765(01)00167-8

実験手法

X-RAY DIFFRACTION (2.37 Å)