1HRS

A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN

1HRS の概要

• エントリーDOI: 10.2210/pdb1hrs/pdb
• 分子名称: APOFERRITIN CO-CRYSTALLIZED WITH SN-PROTOPORPHYRIN IX IN CADMIUM SULFATE, CADMIUM ION, PROTOPORPHYRIN IX, ... (4 entities in total)
• 機能のキーワード: iron storage
• 由来する生物種: Equus caballus (horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20659.91

構造登録者

Precigoux, G.,Yariv, J.,Gallois, B.,Dautant, A.,Courseille, C.,Langlois D'Estaintot, B. (登録日: 1993-11-05, 公開日: 1994-05-31, 最終更新日: 2024-02-07)

主引用文献

Precigoux, G.,Yariv, J.,Gallois, B.,Dautant, A.,Courseille, C.,d'Estaintot, B.L.
A crystallographic study of haem binding to ferritin.
Acta Crystallogr.,Sect.D, 50:739-743, 1994

PubMed Abstract: Ferritin, the iron-storage protein, binds porphyrins, metalloporphyrins and the fluorescent dyes ANS (8-anilino-1-naphthalenesulfonic acid) and TNS (2-p-toluidinyl-6-naphthalenesulfonic acid), similarly to apo-myoglobin. Octahedral crystals of horse-spleen apo-ferritin (HSF; 174 amino acids) complexes prepared by the addition of haem, hematoporphyrin or Sn-protoporphyrin IX to a solution of apo-ferritin crystallize in space group F432 with cell parameter a = 184.0 A. X-ray crystallographic analysis of single crystals prepared from a mixture containing haem or Sn-protoporphyrin IX shows that the haem-binding sites in these crystals are occupied by protoporphyrin IX, which is free of metal, rather than by the original metalloporphyrin. The present paper describes the structure of horse-spleen apo-ferritin cocrystallized with Sn-protoporphyrin IX. The 6797 reflections up to 2.6 A resolution used in the refinement were obtained from a data set recorded on a Nicolet/Xentronics area detector with Cu Kalpha radiation from a Rigaku RU 200 rotating anode. The final structure comprises 1613 non-H atoms, two Cd atoms and 170 solvent molecules. Four residues are described as disordered. The root-mean-square deviations from ideal bond lengths and angles are 0.013 A and 2.88 degrees, respectively. Protoporphyrins are observed in special positions on the twofold axes of the ferritin molecule with a stoichiometry of 0.4 per subunit.

PubMed: 15299370
DOI: 10.1107/S0907444994003227

実験手法

X-RAY DIFFRACTION (2.6 Å)