1HQZ

Cofilin homology domain of a yeast actin-binding protein ABP1P

1HQZ の概要

• エントリーDOI: 10.2210/pdb1hqz/pdb
• 分子名称: ACTIN-BINDING PROTEIN (2 entities in total)
• 機能のキーワード: cofilin homology domain, actin binding, new york sgx research center for structural genomics, nysgxrc, structural genomics, psi, protein structure initiative, structural protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 140745.61

構造登録者

Strokopytov, B.V.,Fedorov, A.A.,Mahoney, N.,Drubin, D.G.,Almo, S.C.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2000-12-20, 公開日: 2001-12-21, 最終更新日: 2023-08-09)

主引用文献

Strokopytov, B.V.,Fedorov, A.,Mahoney, N.M.,Kessels, M.,Drubin, D.G.,Almo, S.C.
Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches.
Acta Crystallogr.,Sect.D, 61:285-293, 2005

PubMed Abstract: A modified molecular-replacement method is described that makes use of six-dimensional searches and the phased translation function, providing a systematic examination of all possible search-model orientations in an experimental electron-density map. As an example, the structure solution of the cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1 (ABP1) is presented in detail. Additional examples are presented in which these tools have significantly aided structure solutions in a variety of contexts. These results suggest that this approach might be of widespread utility for challenging structures involving weak phase information, complex asymmetric units and search models with weak structural homology. Furthermore, this approach supports an exhaustive molecular-replacement strategy in cases where an appropriate search model cannot readily be identified on the basis of sequence homology. The fully automated web-based implementation of this phased translation function is described.

PubMed: 15735338
DOI: 10.1107/S0907444904033037

実験手法

X-RAY DIFFRACTION (2.1 Å)