1HO0

NEW B-CHAIN MUTANT OF BOVINE INSULIN

1HO0 の概要

• エントリーDOI: 10.2210/pdb1ho0/pdb
• 分子名称: INSULIN (1 entity in total)
• 機能のキーワード: beta_turn (20-23), alpha_helix (9-19), hormone-growth factor complex, hormone/growth factor
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3371.80

構造登録者

Dupradeau, F.Y.,Richard, T.,Le Flem, G.,Oulyadi, H.,Prigent, Y.,Monti, J.P. (登録日: 2000-12-08, 公開日: 2000-12-20, 最終更新日: 2024-05-22)

主引用文献

Dupradeau, F.Y.,Richard, T.,Le Flem, G.,Oulyadi, H.,Prigent, Y.,Monti, J.P.
A new B-chain mutant of insulin: comparison with the insulin crystal structure and role of sulfonate groups in the B-chain structure
J.Pept.Res., 60:56-64, 2002

PubMed Abstract: The solution structure of a new B-chain mutant of bovine insulin, in which the cysteines B7 and B19 are replaced by two serines, has been determined by circular dichroism, 2D-NMR and molecular modeling. This structure is compared with that of the oxidized B-chain of bovine insulin [Hawkins et al. (1995) Int. J. Peptide Protein Res.46, 424-433]. Circular dichroism spectroscopy showed in particular that a higher percentage of helical secondary structure for the B-chain mutant is estimated in trifluoroethanol solution in comparison with the oxidized B-chain. 2D-NMR experiments confirmed, among multiple conformations, that the B-chain mutant presents defined secondary structures such as a alpha-helix between residues B9 and B19, and a beta-turn between amino acids B20 and B23 in aqueous trifluoroethanol. The 3D structures, which are consistent with NMR data and were obtained using a simulated annealing protocol, showed that the tertiary structure of the B-chain mutant is better resolved and is more in agreement with the insulin crystal structure than the oxidized B-chain structure described by Hawkins et al. An explanation could be the presence of two sulfonate groups in the oxidized insulin B-chain. Either by their charges and/or their size, such chemical groups could play a destructuring effect and thus could favor peptide flexibility and conformational averaging. Thus, this study provides new insights on the folding of isolated B-chains.

PubMed: 12081626
DOI: 10.1034/j.1399-3011.2002.02990.x

実験手法

SOLUTION NMR