1HMC

THREE-DIMENSIONAL STRUCTURE OF DIMERIC HUMAN RECOMBINANT MACROPHAGE COLONY STIMULATING FACTOR

Summary for 1HMC

• Entry DOI: 10.2210/pdb1hmc/pdb
• Descriptor: HUMAN MACROPHAGE COLONY STIMULATING FACTOR (1 entity in total)
• Functional Keywords: macrophage colony stimulating factor
• Biological source: Homo sapiens (human)
• Cellular location: Cell membrane; Single-pass membrane protein. Processed macrophage colony-stimulating factor 1: Secreted, extracellular space: P09603
• Total number of polymer chains: 2
• Total formula weight: 34585.18

Authors

Bohm, A.,Pandit, J.,Jancarik, J.,Halenbeck, R.,Koths, K.,Kim, S.-H. (deposition date: 1993-12-22, release date: 1994-04-30, Last modification date: 2024-02-07)

Primary citation

Pandit, J.,Bohm, A.,Jancarik, J.,Halenbeck, R.,Koths, K.,Kim, S.H.
Three-dimensional structure of dimeric human recombinant macrophage colony-stimulating factor.
Science, 258:1358-1362, 1992

PubMed Abstract: Macrophage colony-stimulating factor (M-CSF) triggers the development of cells of the monocyte-macrophage lineage and has a variety of stimulatory effects on mature cells of this class. The biologically active form of M-CSF is a disulfide-linked dimer that activates an intrinsic tyrosine kinase activity on the M-CSF receptor by inducing dimerization of the receptor molecules. The structure of a recombinant human M-CSF dimer, determined at 2.5 angstroms by x-ray crystallography, contains two bundles of four alpha helices laid end-to-end, with an interchain disulfide bond. Individual monomers of M-CSF show a close structural similarity to the cytokines granulocyte-macrophage colony-stimulating factor and human growth hormone. Both of these cytokines are monomeric in their active form, and their specific receptors lack intrinsic tyrosine kinase activity. The similarity of these structures suggests that the receptor binding determinants for all three cytokines may be similar.

PubMed: 1455231

Experimental method

X-RAY DIFFRACTION (2.5 Å)