1HMC

THREE-DIMENSIONAL STRUCTURE OF DIMERIC HUMAN RECOMBINANT MACROPHAGE COLONY STIMULATING FACTOR

1HMC の概要

• エントリーDOI: 10.2210/pdb1hmc/pdb
• 分子名称: HUMAN MACROPHAGE COLONY STIMULATING FACTOR (1 entity in total)
• 機能のキーワード: macrophage colony stimulating factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Single-pass membrane protein. Processed macrophage colony-stimulating factor 1: Secreted, extracellular space: P09603
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34585.18

構造登録者

Bohm, A.,Pandit, J.,Jancarik, J.,Halenbeck, R.,Koths, K.,Kim, S.-H. (登録日: 1993-12-22, 公開日: 1994-04-30, 最終更新日: 2024-02-07)

主引用文献

Pandit, J.,Bohm, A.,Jancarik, J.,Halenbeck, R.,Koths, K.,Kim, S.H.
Three-dimensional structure of dimeric human recombinant macrophage colony-stimulating factor.
Science, 258:1358-1362, 1992

PubMed Abstract: Macrophage colony-stimulating factor (M-CSF) triggers the development of cells of the monocyte-macrophage lineage and has a variety of stimulatory effects on mature cells of this class. The biologically active form of M-CSF is a disulfide-linked dimer that activates an intrinsic tyrosine kinase activity on the M-CSF receptor by inducing dimerization of the receptor molecules. The structure of a recombinant human M-CSF dimer, determined at 2.5 angstroms by x-ray crystallography, contains two bundles of four alpha helices laid end-to-end, with an interchain disulfide bond. Individual monomers of M-CSF show a close structural similarity to the cytokines granulocyte-macrophage colony-stimulating factor and human growth hormone. Both of these cytokines are monomeric in their active form, and their specific receptors lack intrinsic tyrosine kinase activity. The similarity of these structures suggests that the receptor binding determinants for all three cytokines may be similar.

PubMed: 1455231

実験手法

X-RAY DIFFRACTION (2.5 Å)