1HKX

Crystal structure of calcium/calmodulin-dependent protein kinase

1HKX の概要

• エントリーDOI: 10.2210/pdb1hkx/pdb
• 関連するPDBエントリー: 1CDM
• 分子名称: CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE TYPE II ALPHA CHAIN, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transferase, serine/threonine-protein kinase, calmodulin-binding, phosphorylation, atp-binding, alternative splicing
• 由来する生物種: MUS MUSCULUS (MOUSE)
• 細胞内の位置: Isoform Alpha KAP: Cytoplasm (Probable): P11798
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 239043.04

構造登録者

Hoelz, A.,Nairn, A.C.,Kuriyan, J. (登録日: 2003-03-12, 公開日: 2003-06-02, 最終更新日: 2024-06-19)

主引用文献

Hoelz, A.,Nairn, A.C.,Kuriyan, J.
Crystal Structure of a Tetradecameric Assembly of the Association Domain of Ca2+/Calmodulin-Dependent Kinase II
Mol.Cell, 11:1241-, 2003

PubMed Abstract: We report the crystal structure of the 143 residue association domain of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII). The association domain forms a hub-like assembly, composed of two rings of seven protomers each, which are stacked head to head and held together by extensive interfaces. The tetradecameric organization of the assembly was confirmed by analytical ultracentrifugation and multiangle light scattering. Individual protomers form wedge-shaped structures from which N-terminal helical segments that connect to the kinase domain extend toward the equatorial plane of the assembly, consistent with the arrangement of the kinase domains in a second outer ring. A deep and highly conserved pocket present within the association domain may serve as a docking site for proteins that interact with CaMKII.

PubMed: 12769848
DOI: 10.1016/S1097-2765(03)00171-0

実験手法

X-RAY DIFFRACTION (2.65 Å)