1HKB

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN BRAIN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND GLUCOSE-6-PHOSPHATE

1HKB の概要

• エントリーDOI: 10.2210/pdb1hkb/pdb
• 分子名称: D-GLUCOSE 6-PHOSPHOTRANSFERASE, beta-D-glucopyranose, 6-O-phosphono-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: phosphotransferase, glycolysis, allosteric enzyme, glucose, glucose-6-phosphate
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 207171.56

構造登録者

Aleshin, A.E.,Zeng, C.,Burenkov, G.P.,Bartunik, H.D.,Fromm, H.J.,Honzatko, R.B. (登録日: 1997-12-01, 公開日: 1998-06-03, 最終更新日: 2024-04-03)

主引用文献

Aleshin, A.E.,Zeng, C.,Bourenkov, G.P.,Bartunik, H.D.,Fromm, H.J.,Honzatko, R.B.
The mechanism of regulation of hexokinase: new insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate.
Structure, 6:39-50, 1998

PubMed Abstract: Hexokinase I is the pacemaker of glycolysis in brain tissue. The type I isozyme exhibits unique regulatory properties in that physiological levels of phosphate relieve potent inhibition by the product, glucose-6-phosphate (Gluc-6-P). The 100 kDa polypeptide chain of hexokinase I consists of a C-terminal (catalytic) domain and an N-terminal (regulatory) domain. Structures of ligated hexokinase I should provide a basis for understanding mechanisms of catalysis and regulation at an atomic level.

PubMed: 9493266
DOI: 10.1016/S0969-2126(98)00006-9

実験手法

X-RAY DIFFRACTION (2.8 Å)