1HJF

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258

Summary for 1HJF

Related1DCS 1E5H 1E5I 1RXF 1RXG 1HJG
DescriptorDEACETOXYCEPHALOSPORIN C SYNTHASE, FE (II) ION, 2-OXO-4-METHYLPENTANOIC ACID, ... (4 entities in total)
Functional Keywordsoxidoreductase, alternative 2-oxoacids, cephem antibiotic biosynthesis, chemical cosubstrate rescue, co-substrate selectivity, 2- oxoglutarate-dependent oxygenase
Biological sourceSTREPTOMYCES CLAVULIGERUS
Total number of polymer chains1
Total molecular weight34748.56
Authors
Lee, H.J.,Lloyd, M.D.,Clifton, I.J.,Harlos, K.,Dubus, A.,Baldwin, J.E.,Frere, J.M.,Schofield, C.J. (deposition date: 2001-01-15, release date: 2001-06-01, Last modification date: 2019-05-08)
Primary citation
Lee, H.J.,Lloyd, M.D.,Harlos, K.,Clifton, I.J.,Baldwin, J.E.,Schofield, C.J.
Alteration of the 2-Oxoacid Cosubstrate Selectivity in Deacetoxycephalosporin C Synthase: The Role of Arginine-258
J.Biol.Chem., 276:18290-, 2001
PubMed: 11279000 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M100085200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.6 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2547 0.4% 1.7% 7.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (1hjf.pdb1.gz [136.1 KB])