1HJD

Melanoma inhibitory activity (MIA) protein

1HJD の概要

• エントリーDOI: 10.2210/pdb1hjd/pdb
• 分子名称: HUMAN MELANOMA INHIBITORY ACTIVITY PROTEIN (1 entity in total)
• 機能のキーワード: growth factor
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11502.13

構造登録者

Stoll, R.,Voelter, W.,Bosserhoff, A.K.,Holak, T.A. (登録日: 2001-01-11, 公開日: 2002-01-29, 最終更新日: 2024-10-16)

主引用文献

Stoll, R.,Renner, C.,Zweckstetter, M.,Bruggert, M.,Ambrosius, D.,Palme, S.,Engh, R.A.,Golob, M.,Breibach, I.,Buettner, R.,Voelter, W.,Holak, T.A.,Bosserhoff, A.K.
The Extracellular Human Melanoma Inhibitory Activity (Mia) Protein Adopts an SH3 Domain-Like Fold.
Embo J., 20:340-, 2001

PubMed Abstract: Melanoma inhibitory activity (MIA) protein is a clinically valuable marker in patients with malignant melanoma, as enhanced values diagnose metastatic melanoma stages III and IV. Here we show that the recombinant human MIA adopts an SH3 domain-like fold in solution, with two perpendicular, antiparallel, three- and five-stranded beta-sheets. In contrast to known structures with the SH3 domain fold, MIA is a single-domain protein, and contains an additional antiparallel beta-sheet and two disulfide bonds. MIA is also the first extracellular protein found to have the SH3 domain-like fold. Furthermore, we show that MIA interacts with fibronectin and that the peptide ligands identified for MIA exhibit a matching sequence to type III human fibronectin repeats, especially to FN14, which is close to an integrin alpha4beta1 binding site. The present study, therefore, may explain the role of MIA in metastasis in vivo, and supports a model in which the binding of human MIA to type III repeats of fibronectin competes with integrin binding, thus detaching cells from the extracellular matrix.

PubMed: 11157741
DOI: 10.1093/EMBOJ/20.3.340

実験手法

SOLUTION NMR