1HE9

Crystal structure of the GAP domain of the Pseudomonas aeruginosa ExoS toxin

1HE9 の概要

• エントリーDOI: 10.2210/pdb1he9/pdb
• 関連するPDBエントリー: 1HE1
• 分子名称: EXOENZYME S (2 entities in total)
• 機能のキーワード: toxin (exoenzyme s), exos, pseudomonas aeruginosa, gap, toxin, virulence factor, signal transduction
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14432.83

構造登録者

Wurtele, M.,Renault, L.,Barbieri, J.T.,Wittinghofer, A.,Wolf, E. (登録日: 2000-11-21, 公開日: 2001-03-19, 最終更新日: 2024-11-13)

主引用文献

Wurtele, M.,Renault, L.,Barbieri, J.T.,Wittinghofer, A.,Wolf, E.
Structure of the Exos Gtpase Activating Domain
FEBS Lett., 491:26-, 2001

PubMed Abstract: Pseudomonas aeruginosa is an opportunistic bacterial pathogen of great medical relevance. One of its major toxins, exoenzyme S (ExoS), is a dual function protein with a C-terminal Ras-ADP-ribosylation domain and an N-terminal GTPase activating protein (GAP) domain specific for Rho-family proteins. We report here the three-dimensional structure of the N-terminal domain of ExoS determined by X-ray crystallography to 2.4 A resolution. Its fold is all helical with a four helix bundle core capped by additional irregular helices. Loops that are known to interact with Rho-family proteins show very large mobility. Considering the importance of ExoS in Pseudomonas pathogenicity, this structure could be of interest for drug targeting.

PubMed: 11226412
DOI: 10.1016/S0014-5793(01)02105-6

実験手法

X-RAY DIFFRACTION (2.4 Å)