1HCW

23-RESIDUE DESIGNED METAL-FREE PEPTIDE BASED ON THE ZINC FINGER DOMAINS, NMR, 35 STRUCTURES

1HCW の概要

• エントリーDOI: 10.2210/pdb1hcw/pdb
• 分子名称: BBA1 (1 entity in total)
• 機能のキーワード: growth response protein, de novo protein design, supersecondary motif
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2739.12

構造登録者

Imperiali, B.,Struthers, M.,Cheng, R.P. (登録日: 1996-09-20, 公開日: 1997-03-12, 最終更新日: 2022-02-23)

主引用文献

Struthers, M.D.,Cheng, R.P.,Imperiali, B.
Design of a monomeric 23-residue polypeptide with defined tertiary structure.
Science, 271:342-345, 1996

PubMed Abstract: Small proteins or protein domains generally require disulfide bridges or metal sites for their stabilization. Here it is shown that the beta beta alpha architecture of zinc fingers can be reproduced in a 23-residue polypeptide in the absence of metal ions. The sequence was obtained through an iterative design process. A key feature of the final design is the incorporation of a type II' beta turn to aid in beta-hairpin formation. Nuclear magnetic resonance analysis reveals that the alpha helix and beta hairpin are held together by a defined hydrophobic core. The availability of this structural template has implications for the development of functional polypeptides.

PubMed: 8553067

実験手法

SOLUTION NMR