1HCR

HIN RECOMBINASE BOUND TO DNA: THE ORIGIN OF SPECIFICITY IN MAJOR AND MINOR GROOVE INTERACTIONS

1HCR の概要

• エントリーDOI: 10.2210/pdb1hcr/pdb
• 分子名称: DNA (5'-D(*TP*GP*TP*TP*TP*TP*TP*GP*AP*TP*AP*AP*GP*A)-3'), DNA (5'-D(*TP*CP*TP*TP*AP*TP*CP*AP*AP*AP*AP*AP*C)-3'), PROTEIN (HIN RECOMBINASE), ... (4 entities in total)
• 機能のキーワード: protein-dna complex, dna binding protein-dna complex, dna binding protein/dna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 14290.49

構造登録者

Feng, J.-A.,Johnson, R.C.,Dickerson, R.E. (登録日: 1993-12-17, 公開日: 1994-04-30, 最終更新日: 2024-02-07)

主引用文献

Feng, J.A.,Johnson, R.C.,Dickerson, R.E.
Hin recombinase bound to DNA: the origin of specificity in major and minor groove interactions.
Science, 263:348-355, 1994

PubMed Abstract: The structure of the 52-amino acid DNA-binding domain of the prokaryotic Hin recombinase, complexed with a DNA recombination half-site, has been solved by x-ray crystallography at 2.3 angstrom resolution. The Hin domain consists of a three-alpha-helix bundle, with the carboxyl-terminal helix inserted into the major groove of DNA, and two flanking extended polypeptide chains that contact bases in the minor groove. The overall structure displays features resembling both a prototypical bacterial helix-turn-helix and the eukaryotic homeodomain, and in many respects is an intermediate between these two DNA-binding motifs. In addition, a new structural motif is seen: the six-amino acid carboxyl-terminal peptide of the Hin domain runs along the minor groove at the edge of the recombination site, with the peptide backbone facing the floor of the groove and side chains extending away toward the exterior. The x-ray structure provides an almost complete explanation for DNA mutant binding studies in the Hin system and for DNA specificity observed in the Hin-related family of DNA invertases.

PubMed: 8278807

実験手法

X-RAY DIFFRACTION (2.3 Å)