1HAO

COMPLEX OF HUMAN ALPHA-THROMBIN WITH A 15MER OLIGONUCLEOTIDE GGTTGGTGTGGTTGG (BASED ON NMR MODEL OF DNA)

1HAO の概要

• エントリーDOI: 10.2210/pdb1hao/pdb
• 関連するPDBエントリー: 1HAP
• 関連するBIRD辞書のPRD_ID: PRD_000020
• 分子名称: DNA 5'-D(*GP*GP*TP*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3', ALPHA-THROMBIN light chain, ALPHA-THROMBIN heavy chain, ... (5 entities in total)
• 機能のキーワード: coagulation, quadruple helix, hydrolase-hydrolase inhibitor-dna complex, hydrolase/hydrolase inhibitor/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted, extracellular space: P00734 P00734
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 39073.79

構造登録者

Tulinsky, A.,Padmanabhan, K. (登録日: 1995-10-03, 公開日: 1996-04-03, 最終更新日: 2024-10-23)

主引用文献

Padmanabhan, K.,Tulinsky, A.
An ambiguous structure of a DNA 15-mer thrombin complex.
Acta Crystallogr.,Sect.D, 52:272-282, 1996

PubMed Abstract: The structure of a complex between thrombin and a GGTTGGTGTGGTTGG DNA 15-mer has been analyzed crystallographically. The solution NMR structure of the 15-mer has two stacked G-quartets similar to that found in the previous X-ray structure determination of the 15-mer-thrombin complex [Padmanabhan, Padmanabhan, Ferrara, Sadler & Tulinsky (1993). J. Biol. Chem. 268, 17651-17654]; the strand polarity, however, is reversed from that of the crystallographic structure. The structure of the complex here has been redetermined with better diffraction data confirming the previous crystallographic structure but also indicating that the NMR solution structure fits equally well. Both 15-mer complex structures refined to an R value of about 0.16 presenting a disconcerting ambiguity. Since the two 15-mer structures associate with thrombin in different ways (through the TGT loop in the X-ray and TT loop in the NMR model), other independent lines of physical or chemical evidence are required to resolve the ambiguity.

PubMed: 15299700
DOI: 10.1107/S0907444995013977

実験手法

X-RAY DIFFRACTION (2.8 Å)