1HAN

CRYSTAL STRUCTURE OF THE BIPHENYL-CLEAVING EXTRADIOL DIOXYGENASE FROM A PCB-DEGRADING PSEUDOMONAD

1HAN の概要

• エントリーDOI: 10.2210/pdb1han/pdb
• 分子名称: 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE, FE (III) ION, TERTIARY-BUTYL ALCOHOL, ... (4 entities in total)
• 機能のキーワード: extradiol dioxygenase, oxidoreductase (oxygenase)
• 由来する生物種: Burkholderia xenovorans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32563.41

構造登録者

Han, S.,Bolin, J.T. (登録日: 1995-08-30, 公開日: 1995-11-14, 最終更新日: 2024-02-07)

主引用文献

Han, S.,Eltis, L.D.,Timmis, K.N.,Muchmore, S.W.,Bolin, J.T.
Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad.
Science, 270:976-980, 1995

PubMed Abstract: Polychlorinated biphenyls (PCBs) typify a class of stable aromatic pollutants that are targeted by bioremediation strategies. In the aerobic degradation of biphenyl by bacteria, the key step of ring cleavage is catalyzed by an Fe(II)-dependent extradiol dioxygenase. The crystal structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB-degrading strain of Pseudomonas cepacia has been determined at 1.9 angstrom resolution. The monomer comprises amino- and carboxyl-terminal domains. Structural homology between and within the domains reveals evolutionary relationships within the extradiol dioxygenase family. The iron atom has five ligands in square pyramidal geometry: one glutamate and two histidine side chains, and two water molecules.

PubMed: 7481800

実験手法

X-RAY DIFFRACTION (1.9 Å)