1HA6

NMR Solution Structure of Murine CCL20/MIP-3a Chemokine

1HA6 の概要

• エントリーDOI: 10.2210/pdb1ha6/pdb
• 分子名称: MACROPHAGE INFLAMMATORY PROTEIN 3 ALPHA (1 entity in total)
• 機能のキーワード: immune system, chemiokine, mouse, ccl20/mip-3a, defensins
• 由来する生物種: MUS MUSCULUS (MOUSE)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7970.39

構造登録者

Perez-Canadillas, J.M.,Zaballos, A.,Gutierrez, J.,Varona, R.,Roncal, F.,Albar, J.P.,Marquez, G.,Bruix, M. (登録日: 2001-03-28, 公開日: 2001-08-22, 最終更新日: 2024-11-20)

主引用文献

Perez-Canadillas, J.M.,Zaballos, A.,Gutierrez, J.,Varona, R.,Roncal, F.,Albar, J.P.,Marquez, G.,Bruix, M.
NMR Solution Structure of Murine Ccl20/Mip3-A, a Chemiokine that Specifically Chemoattracs Immature Dendritic Cells and Lymphocytes Through its Highly Specific Interaction with the Beta-Chemokine Receptor Ccr6
J.Biol.Chem., 276:28372-, 2001

PubMed Abstract: CCL20/MIP-3alpha is a beta-chemokine expressed in the thymus, skin, and intestinal epithelial cells that exclusively binds and activates the CCR6 receptor in both mice and humans. The strict receptor binding specificity of CCL20 is exceptional; other chemokines and their receptors bind promiscuously with multiple partners. Toward determining the structural basis for the selective receptor specificity of CCL20, we have determined its three-dimensional structure by 1H NMR spectroscopy. CCL20 exhibits the same monomeric structure previously described for other chemokines: a three-stranded beta-sheet and an overlying alpha-helix. The CCL20 receptor selectivity could arise from the rigid conformation of the N-terminal DCCL motif as well as the groove between the N-loop and the beta2-beta3 hairpin, which is significantly narrower in CCL20 than in other chemokines. Similar structural features are seen in human beta-defensin 2, a small nonchemokine polypeptide reported to selectively bind and activate CCR6, which stresses their importance for the specific binding of both CCL20 and beta-defensin 2 to CCR6. CCL20's structure will be useful to design tools aimed to modulate its important biological functions.

PubMed: 11373289
DOI: 10.1074/JBC.M103121200

実験手法

SOLUTION NMR