1H98

New Insights into Thermostability of Bacterial Ferredoxins: High Resolution Crystal Structure of the Seven-Iron Ferredoxin from Thermus thermophilus

1H98 の概要

• エントリーDOI: 10.2210/pdb1h98/pdb
• 分子名称: FERREDOXIN, IRON/SULFUR CLUSTER, FE3-S4 CLUSTER, ... (4 entities in total)
• 機能のキーワード: electron transport, thermophilic, iron-sulfur, azotobacter, hydrogen bonds, stability, high resolution
• 由来する生物種: THERMUS AQUATICUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9341.30

構造登録者

Macedo-Ribeiro, S.,Martins, B.M.,Pereira, P.J.B.,Buse, G.,Huber, R.,Soulimane, T. (登録日: 2001-03-05, 公開日: 2001-11-27, 最終更新日: 2023-12-13)

主引用文献

Macedo-Ribeiro, S.,Martins, B.M.,Pereira, P.J.B.,Buse, G.,Huber, R.,Soulimane, T.
New Insights Into the Thermostability of Bacterial Ferredoxins: High-Resolution Crystal Structure of the Seven-Iron Ferredoxin from Thermus Thermophilus
J.Biol.Inorg.Chem., 6:663-, 2001

PubMed Abstract: The crystal structure of the seven-iron ferredoxin from Thermus thermophilus (FdTt) has been determined at 1.64 A resolution, allowing us to unveil the common mechanisms of thermostabilization within "bacterial-type" ferredoxins. FdTt and other homologous thermophilic seven-iron ferredoxins are smaller than their mesophilic counterparts. Thermostabilizing features are optimized in a minimal structural and functional unit, with an extensive cross-linking of secondary structure elements mediated by improved polar and hydrophobic interactions. Most of the potentially stabilizing features are focused on the vicinity of the functional [3Fe-4S] cluster. The structural [4Fe-4S] cluster is shielded in thermophilic FdTt by an increased number of polar interactions involving the two N-terminal residues. Comparisons with the hyperthermostable ferredoxin from Thermotoga maritima reveal that (1) a reduction in the number of non-glycine residues in strained conformations, (2) improved polar interactions within the common iron-sulfur cluster binding (betaalphabeta)2 motif, and (3) an optimized charge distribution at the protein surface, constitute a common strategy for increasing the thermal stability of these ferredoxins.

PubMed: 11681700
DOI: 10.1007/S007750100243

実験手法

X-RAY DIFFRACTION (1.64 Å)