1H6S

Asymmetric conductivity of engineered proteins

1H6S の概要

• エントリーDOI: 10.2210/pdb1h6s/pdb
• 関連するPDBエントリー: 1BH3 1PRN 2PRN 3PRN 5PRN 6PRN 7PRN 8PRN
• 分子名称: PORIN (1 entity in total)
• 機能のキーワード: membrane protein, integral membrane protein porin
• 由来する生物種: RHODOPSEUDOMONAS BLASTICA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32222.72

構造登録者

Bannwarth, M.,Schulz, G.E. (登録日: 2001-06-22, 公開日: 2002-12-12, 最終更新日: 2023-12-13)

主引用文献

Bannwarth, M.,Schulz, G.E.
Asymmetric Conductivity of Engineered Porins
Protein Eng., 15:799-, 2002

PubMed Abstract: Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.

PubMed: 12468713
DOI: 10.1093/PROTEIN/15.10.799

実験手法

X-RAY DIFFRACTION (3 Å)