1H6K

nuclear Cap Binding Complex

1H6K の概要

• エントリーDOI: 10.2210/pdb1h6k/pdb
• 分子名称: CBP80, 20 KDA NUCLEAR CAP BINDING PROTEIN (3 entities in total)
• 機能のキーワード: m7g cap, cap-binding-complex, rnp domain, mif4g domain, rna maturation, rna export, nuclear protein
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 298334.74

構造登録者

Mazza, C.,Ohno, M.,Segref, A.,Mattaj, I.W.,Cusack, S. (登録日: 2001-06-18, 公開日: 2001-09-13, 最終更新日: 2024-05-08)

主引用文献

Mazza, C.,Ohno, M.,Segref, A.,Mattaj, I.W.,Cusack, S.
Crystal Structure of the Human Nuclear CAP Binding Complex
Mol.Cell, 8:383-, 2001

PubMed Abstract: The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises three domains, each containing consecutive helical hairpins and resembling the so-called MIF4G domain found in several other proteins involved in RNA metabolism. The small subunit, CPB20, has an RNP fold and associates with the second and third domains of CBP80. Site-directed mutagenesis revealed 4 residues of CBP20 which are critical for cap binding. A model for cap binding is proposed based on these results and the known mode of binding of RNA to RNP domains.

PubMed: 11545740
DOI: 10.1016/S1097-2765(01)00299-4

実験手法

X-RAY DIFFRACTION (2 Å)