1H5A

STRUCTURE OF FERRIC HORSERADISH PEROXIDASE C1A IN COMPLEX WITH ACETATE

1H5A の概要

• エントリーDOI: 10.2210/pdb1h5a/pdb
• 関連するPDBエントリー: 1ATJ 1H55 1H57 1H58 1H5C 1H5D 1H5E 1H5F 1H5G 1H5H 1H5I 1H5J 1H5K 1H5L 1H5M 1HCH 2ATJ 3ATJ 6ATJ 7ATJ
• 分子名称: PEROXIDASE C1A, PROTOPORPHYRIN IX CONTAINING FE, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, peroxidase, horseradish, ferric state, acetate ion
• 由来する生物種: ARMORACIA RUSTICANA (HORSERADISH)
• 細胞内の位置: Secreted (Probable): P00433
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34762.87

構造登録者

Berglund, G.I.,Carlsson, G.H.,Hajdu, J.,Smith, A.T.,Szoke, H.,Henriksen, A. (登録日: 2001-05-21, 公開日: 2002-06-18, 最終更新日: 2024-11-13)

主引用文献

Berglund, G.I.,Carlsson, G.H.,Smith, A.T.,Szoke, H.,Henriksen, A.,Hajdu, J.
The Catalytic Pathway of Horseradish Peroxidase at High Resolution
Nature, 417:463-, 2002

PubMed Abstract: A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.

PubMed: 12024218
DOI: 10.1038/417463A

実験手法

X-RAY DIFFRACTION (1.6 Å)