1H4Z

Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unphosphorylated Form

1H4Z の概要

• エントリーDOI: 10.2210/pdb1h4z/pdb
• 関連するPDBエントリー: 1H4X 1H4Y
• 分子名称: ANTI-SIGMA F FACTOR ANTAGONIST (2 entities in total)
• 機能のキーワード: cell differentiation, phosphorylation, sigma factor, sporulation
• 由来する生物種: BACILLUS SPHAERICUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13125.22

構造登録者

Seavers, P.R.,Lewis, R.J.,Brannigan, J.A.,Verschueren, K.H.G.,Murshudov, G.N.,Wilkinson, A.J. (登録日: 2001-05-16, 公開日: 2001-07-06, 最終更新日: 2023-12-13)

主引用文献

Seavers, P.R.,Lewis, R.J.,Brannigan, J.A.,Verschueren, K.H.G.,Murshudov, G.N.,Wilkinson, A.J.
Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms
Structure, 9:605-, 2001

PubMed Abstract: The asymmetric cell division during sporulation in Bacillus subtilis gives rise to two compartments: the mother cell and the forespore. Each follow different programs of gene expression coordinated by a succession of alternate RNA polymerase sigma factors. The activity of the first of these sigma factors, sigmaF, is restricted to the forespore although sigmaF is present in the predivisional cell and partitions into both compartments following the asymmetric septation. For sigmaF to become active, it must escape from a complex with its cognate anti-sigma factor, SpoIIAB. This relief from SpoIIAB inhibition requires the dephosphorylation of the anti-sigma factor antagonist, SpoIIAA. The phosphorylation state of SpoIIAA is thus a key determinant of sigmaF activity and cell fate.

PubMed: 11470435
DOI: 10.1016/S0969-2126(01)00623-2

実験手法

X-RAY DIFFRACTION (2.74 Å)