1H3M

Structure of 4-diphosphocytidyl-2C-methyl-D-erythritol synthetase

1H3M の概要

• エントリーDOI: 10.2210/pdb1h3m/pdb
• 関連するPDBエントリー: 1I52 1INI 1INJ
• 分子名称: 2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, CHLORIDE ION, PENTANE-1,5-DIAMINE, ... (4 entities in total)
• 機能のキーワード: transferase, isoprenoid, synthetase
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51621.64

構造登録者

Kemp, L.E.,Bond, C.S.,Hunter, W.N. (登録日: 2002-09-10, 公開日: 2003-08-01, 最終更新日: 2023-12-13)

主引用文献

Kemp, L.E.,Bond, C.S.,Hunter, W.N.
Structure of a Tetragonal Crystal Form of Escherichia Coli 2-C-Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase
Acta Crystallogr.,Sect.D, 59:607-, 2003

PubMed Abstract: 2-C-Methyl-D-erythritol 4-phosphate cytidylyltransferase is an essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis. The structure of a tetragonal crystal form has been solved by molecular replacement and refined to 2.4 A resolution. Structure and sequence comparisons suggest that the enzyme is a suitable target for a structure-based approach to the development of novel broad-spectrum antibiotics. However, the absence of ligands in the enzyme active site together with the moderate resolution of the structure indicates that this tetragonal crystal form is inferior to that of a previously reported highly ordered monoclinic form [Richard et al. (2001), Nature Struct. Biol. 8, 641-647].

PubMed: 12595740
DOI: 10.1107/S090744490202365X

実験手法

X-RAY DIFFRACTION (2.4 Å)