1H2R

THREE-DIMENSIONAL STRUCTURE OF NI-FE HYDROGENASE FROM DESULFIVIBRIO VULGARIS MIYAZAKI F IN THE REDUCED FORM AT 1.4 A RESOLUTION

1H2R の概要

• エントリーDOI: 10.2210/pdb1h2r/pdb
• 分子名称: PROTEIN (PERIPLASMIC [NIFE] HYDROGENASE SMALL SUBUNIT), PROTEIN (PERIPLASMIC [NIFE] HYDROGENASE LARGE SUBUNIT), IRON/SULFUR CLUSTER, ... (7 entities in total)
• 機能のキーワード: high resolution crystal structure, sulfur-bridging ligand, ni-fe hydrogenase, reduced enzyme, atomic cap at active site, oxidoreductase
• 由来する生物種: Desulfovibrio vulgaris str. 'Miyazaki F'; 詳細
• 細胞内の位置: Periplasm: P21853 P21852
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89244.11

構造登録者

Higuchi, Y.,Ogata, H. (登録日: 1999-06-14, 公開日: 2000-01-05, 最終更新日: 2023-08-09)

主引用文献

Higuchi, Y.,Ogata, H.,Miki, K.,Yasuoka, N.,Yagi, T.
Removal of the bridging ligand atom at the Ni-Fe active site of [NiFe] hydrogenase upon reduction with H2, as revealed by X-ray structure analysis at 1.4 A resolution.
Structure Fold.Des., 7:549-556, 1999

PubMed Abstract: The active site of [NiFe] hydrogenase, a heterodimeric protein, is suggested to be a binuclear Ni-Fe complex having three diatomic ligands to the Fe atom and three bridging ligands between the Fe and Ni atoms in the oxidized form of the enzyme. Two of the bridging ligands are thiolate sidechains of cysteinyl residues of the large subunit, but the third bridging ligand was assigned as a non-protein monatomic sulfur species in Desulfovibrio vulgaris Miyazaki F hydrogenase.

PubMed: 10378274
DOI: 10.1016/S0969-2126(99)80071-9

実験手法

X-RAY DIFFRACTION (1.4 Å)