1H1L

NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT

Summary for 1H1L

• Entry DOI: 10.2210/pdb1h1l/pdb
• Related: 1QGU 1QH1 1QH8
• Descriptor: NITROGENASE MOLYBDENUM IRON PROTEIN ALPHA CHAIN, NITROGENASE MOLYBDENUM IRON PROTEIN BETA CHAIN, CITRIC ACID, ... (8 entities in total)
• Functional Keywords: biological nitrogen fixation, nitrogen metabolism, molybdoenzymes, electron transfer, oxidoreductase
• Biological source: KLEBSIELLA PNEUMONIAE; More
• Total number of polymer chains: 4
• Total formula weight: 227960.82

Authors

Mayer, S.M.,Gormal, C.A.,Smith, B.E.,Lawson, D.M. (deposition date: 2002-07-18, release date: 2002-07-29, Last modification date: 2023-12-13)

Primary citation

Mayer, S.M.,Gormal, C.A.,Smith, B.E.,Lawson, D.M.
Crystallographic Analysis of the Mofe Protein of Nitrogenase from a Nifv Mutant of Klebsiella Pneumoniae Identifies Citrate as a Ligand to the Molybdenum of Iron Molybdenum Cofactor (Femoco).
J.Biol.Chem., 277:35263-, 2002

PubMed Abstract: The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase MoFe protein (NifV(-) Kp1) has been determined and refined to a resolution of 1.9 A. This is the first structure for a nitrogenase MoFe protein with an altered cofactor. Moreover, it is the first direct evidence that the organic acid citrate is not just present, but replaces homocitrate as a ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco). Subsequent refinement of the structure revealed that the citrate was present at reduced occupancy.

PubMed: 12133839
DOI: 10.1074/JBC.M205888200

Experimental method

X-RAY DIFFRACTION (1.9 Å)