1H0Q

NMR solution structure of a fully modified locked nucleic acid (LNA) hybridized to RNA

Summary for 1H0Q

• Entry DOI: 10.2210/pdb1h0q/pdb
• Descriptor: 5-D(*(LKC)P*(TLN)P*(LCG)P*(LCA)P*(TLN)P*(LCA)P* (TLN)P*(LCG)P*(LCC))-3, 5-R(*GP*CP*AP*UP*AP*UP*CP*AP*G)-3 (2 entities in total)
• Functional Keywords: dna-rna hybrid, lna, rna, locked nucleic acid, hybrid, dna/rna hybrid
• Total number of polymer chains: 2
• Total formula weight: 5786.74

Authors

Rasmussen, J.,Petersen, M.,Nielsen, K.E.,Kumar, R.,Wengel, J.,Jacobsen, J.P. (deposition date: 2002-06-27, release date: 2003-07-17, Last modification date: 2024-05-15)

Primary citation

Nielsen, K.E.,Rasmussen, J.,Kumar, R.,Wengel, J.,Jacobsen, J.P.,Petersen, M.
NMR Studies of Fully Modified Locked Nucleic Acid (Lna) Hybrids: Solution Structure of an Lna:RNA Hybrid and Characterization of an Lna:RNA Hybrid
Bioconjug.Chem., 15:449-, 2004

PubMed Abstract: LNA is a bicyclic nucleic acid analogue that contains one or more 2'-O,4'-C methylene linkage(s), which effectively locks the furanose ring in a C3'-endo conformation. We report here the NMR solution structure of a nonamer LNA:RNA hybrid and a structural characterization of a nonamer LNA:DNA hybrid, where the LNA strands are composed entirely of LNA nucleotides. This is the first structural characterization of fully modified LNA oligonucleotides. The high-resolution structure reveals that the LNA:RNA hybrid adopts an almost canonical A-type duplex morphology. The helix axis is almost straight and the duplex geometry is regular. This shows that fully modified LNA oligomers can hybridize with complementary RNA and form duplexes within the Watson-Crick framework. The LNA:DNA hybrid structurally resembles an RNA:DNA hybrid as shown by determination of deoxyribose sugar puckers and analysis of NOESY NMR spectra.

PubMed: 15149171
DOI: 10.1021/BC034145H

Experimental method

SOLUTION NMR