1H0H

Tungsten containing Formate Dehydrogenase from Desulfovibrio Gigas

1H0H の概要

• エントリーDOI: 10.2210/pdb1h0h/pdb
• 分子名称: FORMATE DEHYDROGENASE SUBUNIT ALPHA, FORMATE DEHYDROGENASE SUBUNIT BETA, TUNGSTEN ION, ... (10 entities in total)
• 機能のキーワード: electron transport, tungsten selenium formate dehydrogenase, selenocysteine, molybdopterin, mgd, iron-sulphur cluster, periplasmic
• 由来する生物種: DESULFOVIBRIO GIGAS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 274267.64

構造登録者

Raaijmakers, H.C.A. (登録日: 2002-06-19, 公開日: 2003-02-20, 最終更新日: 2024-10-23)

主引用文献

Raaijmakers, H.C.A.,Macieira, S.,Dias, J.,Teixeira, S.,Bursakov, S.,Huber, R.,Moura, J.,Moura, I.,Romao, M.
Gene Sequence and the 1.8 A Crystal Structure of the Tungsten-Containing Formate Dehydrogenase from Desulfovibrio Gigas
Structure, 10:1261-, 2002

PubMed Abstract: Desulfovibrio gigas formate dehydrogenase is the first representative of a tungsten-containing enzyme from a mesophile that has been structurally characterized. It is a heterodimer of 110 and 24 kDa subunits. The large subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase, harbors the W site and one [4Fe-4S] center. No small subunit ortholog containing three [4Fe-4S] clusters has been reported. The structural homology with E. coli FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the active site are conserved. The active site is accessible via a positively charged tunnel, while product release may be facilitated, for H(+) by buried waters and protonable amino acids and for CO(2) through a hydrophobic channel.

PubMed: 12220497
DOI: 10.1016/S0969-2126(02)00826-2

実験手法

X-RAY DIFFRACTION (1.8 Å)