1GYD

Structure of Cellvibrio cellulosa alpha-L-arabinanase

1GYD の概要

• エントリーDOI: 10.2210/pdb1gyd/pdb
• 関連するPDBエントリー: 1GYE 1GYH
• 分子名称: ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE A (2 entities in total)
• 機能のキーワード: arabinanase, hydrolase, propeller, catalysis, cellvibrio, pseudomonas
• 由来する生物種: CELLVIBRIO CELLULOSA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35933.21

構造登録者

Nurizzo, D.,Turkenburg, J.P.,Charnock, S.J.,Roberts, S.M.,Dodson, E.J.,McKie, V.A.,Taylor, E.J.,Gilbert, H.J.,Davies, G.J. (登録日: 2002-04-23, 公開日: 2002-08-23, 最終更新日: 2024-10-16)

主引用文献

Nurizzo, D.,Turkenburg, J.P.,Charnock, S.J.,Roberts, S.M.,Dodson, E.J.,McKie, V.A.,Taylor, E.J.,Gilbert, H.J.,Davies, G.J.
Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold.
Nat. Struct. Biol., 9:665-668, 2002

PubMed Abstract: Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme known to display this topology. A long V-shaped surface groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Three carboxylates deep in the active site groove provide the general acid and base components for glycosidic bond hydrolysis with inversion of anomeric configuration.

PubMed: 12198486
DOI: 10.1038/nsb835

実験手法

X-RAY DIFFRACTION (2.05 Å)