1GXI

PsaE Subunit of the Photosystem I of the Cyanobacterium Synechocystis sp. PCC 6803

1GXI の概要

• エントリーDOI: 10.2210/pdb1gxi/pdb
• 分子名称: PHOTOSYSTEM I REACTION CENTER SUBUNIT IV (1 entity in total)
• 機能のキーワード: photosynthesis, photosystem i, psae sub-unit, thylakoid
• 由来する生物種: SYNECHOCYSTIS SP.
• 細胞内の位置: Cellular thylakoid membrane ; Peripheral membrane protein : P12975
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8022.89

構造登録者

Barth, P.,Savarin, P.,Gilquin, B.,Lagoutte, B.,Ochsenbein, F. (登録日: 2002-04-05, 公開日: 2003-04-04, 最終更新日: 2024-05-15)

主引用文献

Barth, P.,Savarin, P.,Gilquin, B.,Lagoutte, B.,Ochsenbein, F.
Solution NMR Structure and Backbone Dynamics of the Psae Subunit of Photosyetm I from Synechocystis Sp. Pcc 6803
Biochemistry, 41:13902-13914, 2002

PubMed Abstract: PsaE is a small peripheral subunit of photosystem I (PSI) that is very accessible to the surrounding medium. It plays an essential role in optimizing the interactions with the soluble electron acceptors of PSI, ferredoxin and flavodoxin. The solution structure of PsaE from the cyanobacterium Synechocystis sp. PCC 6803 has been investigated by NMR with a special emphasis on its protein dynamic properties. PsaE is characterized by a well-defined central core that consists of a five-stranded beta-sheet (+1, +1, +1, -4x). Four loops (designated the A-B, B-C, C-D, and D-E loops) connect these beta-strands, the overall resulting structure being that of an SH3-like domain. As compared to previously determined PsaE structures, conformational differences are observed in the first three loops. The flexibility of the loops was investigated using (15)N relaxation experiments. This flexibility is small in amplitude for the A-B and B-C loops, but is large for the C-D loop, particularly in the region corresponding to the missing sequence of Nostoc sp. PCC 8009. The plasticity of the connecting loops in the free subunit is compared to that when bound to the PSI and discussed in relation to the insertion process and the function(s) of PsaE.

PubMed: 12437347
DOI: 10.1021/BI0259599

実験手法

SOLUTION NMR