1GWP

STRUCTURE OF THE N-TERMINAL DOMAIN OF THE MATURE HIV-1 CAPSID PROTEIN

「1GDS」から置き換えられました 「1GDY」から置き換えられました 「1GDZ」から置き換えられました

1GWP の概要

• エントリーDOI: 10.2210/pdb1gwp/pdb
• 関連するPDBエントリー: 1A43 1A8O 1AFV 1AK4 1AUM 1BAJ 1HIW 2HMX
• 分子名称: GAG POLYPROTEIN (1 entity in total)
• 機能のキーワード: human immunodeficiency virus type 1 capsid protein, amimo-terminal core domain, hiv-1 ca-151, virus capsid protein, virus maturation, viral protein
• 由来する生物種: HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (NEW YORK-5 ISOLATE)
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential): P12493
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16716.19

構造登録者

Tang, C.,Gitti, R.K.,Lee, B.M.,Walker, J.,Summers, M.F.,Yoo, S.,Sundquist, W.I. (登録日: 2002-03-22, 公開日: 2002-06-21, 最終更新日: 2024-05-15)

主引用文献

Tang, C.,Ndassa, Y.,Summers, M.F.
Structure of the N-Terminal 283-Residue Fragment of the Immature HIV-1 Gag Polyprotein
Nat.Struct.Biol., 9:537-, 2002

PubMed Abstract: The capsid protein (CA) of the mature human immunodeficiency virus (HIV) contains an N-terminal beta-hairpin that is essential for formation of the capsid core particle. CA is generated by proteolytic cleavage of the Gag precursor polyprotein during viral maturation. We have determined the NMR structure of a 283-residue N-terminal fragment of immature HIV-1 Gag (Gag(283)), which includes the intact matrix (MA) and N-terminal capsid (CA(N)) domains. The beta-hairpin is unfolded in Gag(283), consistent with the proposal that hairpin formation occurs subsequent to proteolytic cleavage of Gag, triggering capsid assembly. Comparison of the immature and mature CA(N) structures reveals that beta-hairpin formation induces a approximately 2 A displacement of helix 6 and a concomitant displacement of the cyclophylin-A (CypA)-binding loop, suggesting a possible allosteric mechanism for CypA-mediated destabilization of the capsid particle during infectivity.

PubMed: 12032547
DOI: 10.1038/NSB806

実験手法

SOLUTION NMR