1GUC

SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES

1GUC の概要

• エントリーDOI: 10.2210/pdb1guc/pdb
• 分子名称: RNA (5'-R(*GP*AP*GP*GP*UP*CP*UP*C)-3') (1 entity in total)
• 機能のキーワード: ribonucleic acid, g:u mismatch, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5085.12

構造登録者

Mcdowell, J.A.,Turner, D.H. (登録日: 1996-08-23, 公開日: 1997-02-12, 最終更新日: 2024-05-22)

主引用文献

McDowell, J.A.,Turner, D.H.
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing.
Biochemistry, 35:14077-14089, 1996

PubMed Abstract: The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.

PubMed: 8916893
DOI: 10.1021/bi9615710

実験手法

SOLUTION NMR