1GT7

L-rhamnulose-1-phosphate aldolase from Escherichia coli

1GT7 の概要

• エントリーDOI: 10.2210/pdb1gt7/pdb
• 分子名称: RHAMNULOSE-1-PHOSPHATE ALDOLASE, ZINC ION, PHOSPHOGLYCOLOHYDROXAMIC ACID, ... (4 entities in total)
• 機能のキーワード: lyase, aldolase (lyase), class ii, zinc enzyme, bacterial l- rhamnose metabolism, cleavage of l-rhamnulose-1-phosphate to dihydroxyacetonephosphate and l-lactaldehyde
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 608217.18

構造登録者

Kroemer, M.,Schulz, G.E. (登録日: 2002-01-14, 公開日: 2002-05-03, 最終更新日: 2024-05-08)

主引用文献

Kroemer, M.,Schulz, G.E.
The Structure of L-Rhamnulose-1-Phosphate Aldolase (Class II) Solved by Low-Resolution Sir Phasing and 20-Fold Ncs Averaging
Acta Crystallogr.,Sect.D, 58:824-, 2002

PubMed Abstract: The enzyme L-rhamnulose-1-phosphate aldolase catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate and L-lactaldehyde. It is a homotetramer with an M(r) of 30 000 per subunit and crystallized in space group P3(2)21. The enzyme shows a low sequence identity of 18% with the structurally known L-fuculose-1-phosphate aldolase that splits a stereoisomer in a similar reaction. Structure analysis was initiated with a single heavy-atom derivative measured to 6 A resolution. The resulting poor electron density, a self-rotation function and the working hypothesis that both enzymes are C(4) symmetric with envelopes that resemble one another allowed the location of the 20 protomers of the asymmetric unit. The crystal-packing unit was a D(4)-symmetric propeller consisting of five D(4)-symmetric octamers around an internal crystallographic twofold axis. Presumably, the propellers associate laterally in layers, which in turn pile up along the 3(2) axis to form the crystal. The non-crystallographic symmetry was used to extend the phases to the 2.7 A resolution limit and to establish a refined atomic model of the enzyme. The structure showed that the two enzymes are indeed homologous and that they possess chemically similar active centres.

PubMed: 11976494
DOI: 10.1107/S0907444902004614

実験手法

X-RAY DIFFRACTION (2.7 Å)