1GQK

Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid

1GQK の概要

• エントリーDOI: 10.2210/pdb1gqk/pdb
• 関連するPDBエントリー: 1GQI 1GQJ 1GQL
• 分子名称: ALPHA-D-GLUCURONIDASE, beta-D-glucopyranuronic acid, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: hydrolase, glucuronidase, (alpha-beta)8 barrel, glycoside hydrolase, glucuronic acid
• 由来する生物種: CELLVIBRIO JAPONICUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 162483.75

構造登録者

Nurizzo, D.,Nagy, T.,Gilbert, H.J.,Davies, G.J. (登録日: 2001-11-26, 公開日: 2002-09-26, 最終更新日: 2024-05-01)

主引用文献

Nurizzo, D.,Nagy, T.,Gilbert, H.J.,Davies, G.J.
The Structural Basis for Catalysis and Specificity of the Pseudomonas Cellulosa Alpha-Glucuronidase, Glca67A
Structure, 10:547-, 2002

PubMed Abstract: Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. The structure of the alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three domains, the central of which is a (beta/alpha)(8) barrel housing the catalytic apparatus. Complexes of the enzyme with the individual reaction products, either xylobiose or glucuronic acid, and the ternary complex of both glucuronic acid and xylotriose reveal a "blind" pocket which selects for short decorated xylooligosaccharides substituted with the uronic acid at their nonreducing end, consistent with kinetic data. The catalytic center reveals a constellation of carboxylates; Glu292 is poised to provide protonic assistance to leaving group departure with Glu393 and Asp365 both appropriately positioned to provide base-catalyzed assistance for inverting nucleophilic attack by water.

PubMed: 11937059
DOI: 10.1016/S0969-2126(02)00742-6

実験手法

X-RAY DIFFRACTION (1.9 Å)