1GNZ

LECTIN I-B4 FROM GRIFFONIA SIMPLICIFOLIA (GS I-B4)METAL FREE FORM

1GNZ の概要

• エントリーDOI: 10.2210/pdb1gnz/pdb
• 関連するPDBエントリー: 1GSL 1LEC 1LED
• 分子名称: GSI-B4 ISOLECTIN, PHOSPHATE ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: glycoprotein, sugar binding protein
• 由来する生物種: GRIFFONIA SIMPLICIFOLIA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28962.60

構造登録者

Lescar, J.,Loris, R.,Mitchell, E.,Gautier, C.,Imberty, A. (登録日: 2001-10-11, 公開日: 2001-11-29, 最終更新日: 2024-11-20)

主引用文献

Lescar, J.,Loris, R.,Mitchell, E.,Gautier, C.,Chazalet, V.,Cox, V.,Wyns, L.,Perez, S.,Breton, C.,Imberty, A.
Isolectins I-A and I-B of Griffonia (Bandeiraea) Simplicifolia. Crystal Structure of Metal-Free Gs I-B(4) and Molecular Basis for Metal Binding and Monosaccharide Specificity.
J.Biol.Chem., 277:6608-, 2002

PubMed Abstract: Seeds from the African legume shrub Griffonia simplicifolia contain several lectins. Among them the tetrameric lectin GS I-B(4) has strict specificity for terminal alpha Gal residues, whereas the closely related lectin GS I-A(4) can also bind to alpha GalNAc. These two lectins are commonly used as markers in histology or for research in xenotransplantation. To elucidate the basis for the fine difference in specificity, the amino acid sequences of both lectins have been determined and show 89% identity. The crystal structure of GS I-B(4), determined at 2.5-A resolution, reveals a new quaternary structure that has never been observed in other legume lectins. An unexpected loss of both Ca(2+) and Mn(2+) ions, which are necessary for carbohydrate binding in legume lectins, may be related to a particular amino acid sequence Pro-Glu-Pro in the metal binding loop. Comparison with demetallized concanavalin A reveals a different process for the loss of metal ions and for the subsequent loss of carbohydrate binding activity. The GS I-A x alpha GalNAc and GS I-B x alpha Gal complexes were constructed using homology modeling and docking approaches. The unusual presence of an aromatic amino acid at position 47 (Tyr in I-A and Trp in I-B) explains the strong preference for alpha-anomeric sugars in both isolectins. Alteration at one amino acid position, Ala(106) in I-A versus Glu(106) in I-B, is the basis for the observed specificities toward alpha GalNAc and alpha Gal.

PubMed: 11714720
DOI: 10.1074/JBC.M109867200

実験手法

X-RAY DIFFRACTION (2.5 Å)