1GLC

CATION PROMOTED ASSOCIATION (CPA) OF A REGULATORY AND TARGET PROTEIN IS CONTROLLED BY PHOSPHORYLATION

1GLC の概要

• エントリーDOI: 10.2210/pdb1glc/pdb
• 分子名称: GLUCOSE-SPECIFIC PROTEIN IIIGlc, GLYCEROL KINASE, ZINC ION, ... (7 entities in total)
• 機能のキーワード: phosphotransferase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm : P69783
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74991.16

構造登録者

Feese, M.D.,Meadow, N.D.,Roseman, S.,Pettigrew, D.W.,Remington, S.J. (登録日: 1994-03-07, 公開日: 1994-05-31, 最終更新日: 2024-02-07)

主引用文献

Feese, M.,Pettigrew, D.W.,Meadow, N.D.,Roseman, S.,Remington, S.J.
Cation-promoted association of a regulatory and target protein is controlled by protein phosphorylation.
Proc.Natl.Acad.Sci.USA, 91:3544-3548, 1994

PubMed Abstract: A central question in molecular biology concerns the means by which a regulatory protein recognizes different targets. IIIGlc, the glucose-specific phosphocarrier protein of the bacterial phosphotransferase system, is also the central regulatory element of the PTS. Binding of unphosphorylated IIIGlc inhibits several non-PTS proteins, but there is little or no sequence similarity between IIIGlc binding sites on different target proteins. The crystal structure of Escherichia coli IIIGlc bound to one of its regulatory targets, glycerol kinase, has been refined at 2.6-A resolution in the presence of products, adenosine diphosphate and glycerol 3-phosphate. Structural and kinetic analyses show that the complex of IIIGlc with glycerol kinase creates an intermolecular Zn(II) binding site with ligation identical to that of the zinc peptidase thermolysin. The zinc is coordinated by the two active-site histidines of IIIGlc, a glutamate of glycerol kinase, and a water molecule. Zn(II) at 0.01 and 0.1 mM decreases the Ki of IIIGlc for glycerol kinase by factors of about 15 and 60, respectively. The phosphorylation of one of the histidines of IIIGlc, in its alternative role as phosphocarrier, provides an elegant means of controlling the cation-enhanced protein-protein regulatory interaction. The need for the target protein to supply only one metal ligand may account for the lack of sequence similarity among the regulatory targets of IIIGlc.

PubMed: 8170944
DOI: 10.1073/pnas.91.9.3544

実験手法

X-RAY DIFFRACTION (2.65 Å)