1GL5

NMR structure of the SH3 domain from the Tec protein tyrosine kinase

1GL5 の概要

• エントリーDOI: 10.2210/pdb1gl5/pdb
• 分子名称: TYROSINE-PROTEIN KINASE TEC (1 entity in total)
• 機能のキーワード: transferase, tyrosine-protein kinase, atp-binding, sh3 domain, phosphorylation
• 由来する生物種: MUS MUSCULUS (MOUSE)
• 細胞内の位置: Cytoplasm: P24604
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7908.66

構造登録者

Mulhern, T.D.,Pursglove, S.E.,Booker, G.W. (登録日: 2001-08-28, 公開日: 2001-11-28, 最終更新日: 2024-05-15)

主引用文献

Pursglove, S.E.,Mulhern, T.D.,Mackay, J.P.,Hinds, M.G.,Booker, G.W.
The solution structure and intramolecular associations of the Tec kinase SRC homology 3 domain.
J. Biol. Chem., 277:755-762, 2002

PubMed Abstract: Tec is the prototypic member of a family of intracellular tyrosine kinases that includes Txk, Bmx, Itk, and Btk. Tec family kinases share similarities in domain structure with Src family kinases, but one of the features that differentiates them is a proline-rich region (PRR) preceding their Src homology (SH) 3 domain. Evidence that the PRR of Itk can bind in an intramolecular fashion to its SH3 domain and the lack of a regulatory tyrosine in the C terminus indicates that Tec kinases must be regulated by a different set of intramolecular interactions to the Src kinases. We have determined the solution structure of the Tec SH3 domain and have investigated interactions with its PRR, which contains two SH3-binding sites. We demonstrate that in vitro, the Tec PRR can bind in an intramolecular fashion to the SH3. However, the affinity is lower than that for dimerization via reciprocal PRR-SH3 association. Using site-directed mutagenesis we show that both sites can bind the Tec SH3 domain; site 1 (155KTLPPAP161) binds intramolecularly, while site 2 (165KRRPPPPIPP174) cannot and binds in an intermolecular fashion. These distinct roles for the SH3 binding sites in Tec family kinases could be important for protein targeting and enzyme activation.

PubMed: 11684687
DOI: 10.1074/jbc.M108318200

実験手法

SOLUTION NMR