1GJS

Solution structure of the Albumin binding domain of Streptococcal Protein G

1GJS の概要

• エントリーDOI: 10.2210/pdb1gjs/pdb
• 関連するPDBエントリー: 1FCC 1FCL 1FD6 1GB4 1GJT 1IBX 1P7E 1P7F 2IGG
• 分子名称: IMMUNOGLOBULIN G BINDING PROTEIN G (1 entity in total)
• 機能のキーワード: immunoglobulin-binding protein, bacterial surface protein, albumin binding, protein g
• 由来する生物種: STREPTOCOCCUS SP. (GROUP G STREPTOCOCCI)
• 細胞内の位置: Secreted, cell wall ; Peptidoglycan-anchor : P19909
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7111.09

構造登録者

Johansson, M.U.,Frick, I.M.,Nilsson, H.,Kraulis, P.J.,Hober, S.,Jonasson, P.,Nygren, A.P.,Uhlen, M.,Bjorck, L.,Drakenberg, T.,Forsen, S.,Wikstrom, M. (登録日: 2001-08-02, 公開日: 2001-08-09, 最終更新日: 2024-05-15)

主引用文献

Johansson, M.,Frick, I.,Nilsson, H.,Kraulis, P.,Hober, S.,Jonasson, P.,Linhult, M.,Nygren, P.,Uhlen, M.,Bjorck, L.,Drakenberg, T.,Forsen, S.,Wikstrom, M.
Structure, Specificity, and Mode of Interaction for Bacterial Albumin-Binding Modules
J.Biol.Chem., 277:8114-, 2002

PubMed Abstract: We have determined the solution structure of an albumin binding domain of protein G, a surface protein of group C and G streptococci. We find that it folds into a left handed three-helix bundle similar to the albumin binding domain of protein PAB from Peptostreptococcus magnus. The two domains share 59% sequence identity, are thermally very stable, and bind to the same site on human serum albumin. The albumin binding site, the first determined for this structural motif known as the GA module, comprises residues spanning the first loop to the beginning of the third helix and includes the most conserved region of GA modules. The two GA modules have different affinities for albumin from different species, and their albumin binding patterns correspond directly to the host specificity of C/G streptococci and P. magnus, respectively. These studies of the evolution, structure, and binding properties of the GA module emphasize the power of bacterial adaptation and underline ecological and medical problems connected with the use of antibiotics.

PubMed: 11751858
DOI: 10.1074/JBC.M109943200

実験手法

SOLUTION NMR