1GJH

HUMAN BCL-2, ISOFORM 2

「1G5O」から置き換えられました

1GJH の概要

• エントリーDOI: 10.2210/pdb1gjh/pdb
• 関連するPDBエントリー: 1BXL 1G5J 1G5M
• 分子名称: PROTEIN (APOPTOSIS REGULATOR BCL-2 WITH PUTATIVE FLEXIBLE LOOP REPLACED WITH A PORTION OF APOPTOSIS REGULATOR BCL-X PROTEIN) (1 entity in total)
• 機能のキーワード: apoptosis
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion outer membrane; Single-pass membrane protein: P10415
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19357.56

構造登録者

Petros, A.M.,Medek, A.,Nettesheim, D.G.,Kim, D.H.,Yoon, H.S.,Swift, K.,Matayoshi, E.D.,Oltersdorf, T.,Fesik, S.W. (登録日: 2001-05-31, 公開日: 2001-06-13, 最終更新日: 2023-12-27)

主引用文献

Petros, A.M.,Medek, A.,Nettesheim, D.G.,Kim, D.H.,Yoon, H.S.,Swift, K.,Matayoshi, E.D.,Oltersdorf, T.,Fesik, S.W.
Solution structure of the antiapoptotic protein bcl-2.
Proc.Natl.Acad.Sci.USA, 98:3012-3017, 2001

PubMed Abstract: The structures of two isoforms of Bcl-2 that differ by two amino acids have been determined by NMR spectroscopy. Because wild-type Bcl-2 behaved poorly in solution, the structures were determined by using Bcl-2/Bcl-x(L) chimeras in which part of the putative unstructured loop of Bcl-2 was replaced with a shortened loop from Bcl-x(L). These chimeric proteins have a low pI compared with the wild-type protein and are soluble. The structures of the two Bcl-2 isoforms consist of 6 alpha-helices with a hydrophobic groove on the surface similar to that observed for the homologous protein, Bcl-x(L). Comparison of the Bcl-2 structures to that of Bcl-x(L) shows that although the overall fold is the same, there are differences in the structural topology and electrostatic potential of the binding groove. Although the structures of the two isoforms of Bcl-2 are virtually identical, differences were observed in the ability of the proteins to bind to a 25-residue peptide from the proapoptotic Bad protein and a 16-residue peptide from the proapoptotic Bak protein. These results suggest that there are subtle differences in the hydrophobic binding groove in Bcl-2 that may translate into differences in antiapoptotic activity for the two isoforms.

PubMed: 11248023
DOI: 10.1073/pnas.041619798

実験手法

SOLUTION NMR