1GIO

NMR SOLUTION STRUCTURE OF BOVINE ANGIOGENIN, 10 STRUCTURES

1GIO の概要

• エントリーDOI: 10.2210/pdb1gio/pdb
• 分子名称: ANGIOGENIN (1 entity in total)
• 機能のキーワード: endoribonuclease, hydrolase angiogenesis
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P10152
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14621.51

構造登録者

Lequin, O.,Albaret, C.,Bontems, F.,Spik, G.,Lallemand, J.Y. (登録日: 1996-04-12, 公開日: 1996-12-07, 最終更新日: 2024-10-30)

主引用文献

Lequin, O.,Albaret, C.,Bontems, F.,Spik, G.,Lallemand, J.Y.
Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy.
Biochemistry, 35:8870-8880, 1996

PubMed Abstract: The three-dimensional structure of bovine angiogenin has been determined using two- and three-dimensional proton NMR spectroscopy. The solution structure is very close to that recently determined by X-ray diffraction analysis. This structure appears well defined, even if five loops and one helix exhibit greater flexibility. Analysis of the active site geometry confirms the position of the Glu-118 residue which obstructs the pyrimidine binding site. There is no experimental evidence of an unobstructed conformation of angiogenin in solution. In addition, it appears that the Glu-118 and Ser-119 residues and the cell receptor binding loop may play an important role in the differences of C-terminal fragment organization and ribonucleolytic activity observed between angiogenins and ribonuclease A.

PubMed: 8688423
DOI: 10.1021/bi960022r

実験手法

SOLUTION NMR