1GIF

HUMAN GLYCOSYLATION-INHIBITING FACTOR

1GIF の概要

• エントリーDOI: 10.2210/pdb1gif/pdb
• 分子名称: GLYCOSYLATION-INHIBITING FACTOR (2 entities in total)
• 機能のキーワード: macrophage, inflammatory response, cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P14174
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37458.76

構造登録者

Kato, Y.,Kuroki, R. (登録日: 1996-02-27, 公開日: 1997-03-12, 最終更新日: 2024-02-07)

主引用文献

Kato, Y.,Muto, T.,Tomura, T.,Tsumura, H.,Watarai, H.,Mikayama, T.,Ishizaka, K.,Kuroki, R.
The crystal structure of human glycosylation-inhibiting factor is a trimeric barrel with three 6-stranded beta-sheets.
Proc.Natl.Acad.Sci.USA, 93:3007-3010, 1996

PubMed Abstract: Glycosylation-inhibiting factor (GIF) is a cytokine that is involved in the regulation of IgE synthesis. The crystal structure of recombinant human GIF was determined by the multiple isomorphous replacement method. The structure was refined to an R factor of 0.168 at 1.9 angstrom resolution. The overall structure is seen to consist of three interconnected subunits forming a barrel with three 6-stranded beta-sheets on the inside and six alpha-helices on the outside. There is a 5-angstrom-diameter "hole" through the middle of the barrel. The barrel structure of GIF in part resembles other "trefoil" cytokines such as interleukin 1 and fibroblast growth factor. Each subunit has a new class of alpha + beta sandwich structure consisting of two beta-alpha-beta motifs. These beta-alpha-beta motifs are related by a pseudo-twofold axis and resemble both interleukin 8 and the peptide binding domain of major histocompatibility complex protein, although the topology of the polypeptide chain is quite different.

PubMed: 8610159
DOI: 10.1073/pnas.93.7.3007

実験手法

X-RAY DIFFRACTION (1.9 Å)