1GID

CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING

Summary for 1GID

• Entry DOI: 10.2210/pdb1gid/pdb
• Descriptor: P4-P6 RNA RIBOZYME DOMAIN, MAGNESIUM ION, COBALT HEXAMMINE(III), ... (4 entities in total)
• Functional Keywords: rna, p4-p6 ribozyme domain of the tetrahymena group i intron, ribozyme
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 103330.43

Authors

Cate, J.H.,Gooding, A.R.,Podell, E.,Zhou, K.,Golden, B.L.,Kundrot, C.E.,Cech, T.R.,Doudna, J.A. (deposition date: 1996-08-22, release date: 1996-12-31, Last modification date: 2024-02-07)

Primary citation

Cate, J.H.,Gooding, A.R.,Podell, E.,Zhou, K.,Golden, B.L.,Kundrot, C.E.,Cech, T.R.,Doudna, J.A.
Crystal structure of a group I ribozyme domain: principles of RNA packing.
Science, 273:1678-1685, 1996

PubMed Abstract: Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome.

PubMed: 8781224

Experimental method

X-RAY DIFFRACTION (2.5 Å)